Abberior Instruments STED/RESOLFT

Super resolution microscopy

Location

CIID, EG, Room 045

Spatial Light Modulator (SLM) 2D/3D STED (resolution down to 25nm in 2D and 75nm in 3D)

Adaptive Illumination (RESCue, DyMIN)

Environment Control

Hardware-based autofocus

Lasers:

405nm, cw, 50mW

488nm, cw, 50mW

594nm, pulsed, 300uW pulse@40MHz

640nm, pulsed, 1mW pulse@40MHz

775nm STED laser, pulsed, 3W@40MHz

Detection:

3 APDs (filters 525/50, 615/20, 685/70)

Objectives:

Olympus 40x, UPLFLN, 1.3NA Oil, 0.2mm WD

Olympus 60x, UPLSAPO 1.2NA water, 0.28mm WD

Olympus 100x, UPLSAPO 1.4NA Oil, 0.13mm WD

Book the system here