Abberior Instruments STED/RESOLFT

Super resolution microscopy

Location

CIID, BSL2, EG, Room 045

Spatial Light Modulator (SLM) 2D/3D STED (resolution down to 25nm in 2D and 75nm in 3D)

Adaptive Illumination (RESCue, DyMIN)

Environment Control

Hardware-based autofocus

Lasers:

405nm, cw, 50mW

488nm, cw, 50mW

594nm, pulsed, 300uW pulse@40MHz

640nm, pulsed, 1mW pulse@40MHz

775nm STED laser, pulsed, 3W@40MHz

Detection:

3 APDs (filters 525/50, 615/20, 685/70)

Objectives:

Olympus 40x, UPLFLN, 1.3NA Oil, 0.2mm WD

Olympus 60x, UPLSAPO 1.2NA water, 0.28mm WD

Olympus 100x, UPLSAPO 1.4NA Oil, 0.13mm WD

Book the system here

Click here for materials and methods template