Abberior Instruments STED/FLIM
Super resolution microscopy
Location
CIID, BSL2, EG, Room 045
Spatial Light Modulator (SLM) 2D/3D STED (resolution down to 25nm in 2D and 75nm in 3D)
Adaptive Illumination (RESCue, DyMIN)
Environment Control
Lasers:
405nm, cw, 50mW
485nm, pulsed, 1mW@40MHz
561nm, pulsed, 200uW@40MHz
640nm, pulsed, 1mW@40MHz
775nm STED laser, pulsed, 3W@40MHz
Detection:
3 APDs (spectral detection)
Fluorescence Lifetime Imaging Microscopy (FLIM)
FLIM hardware MultiHarp 150 P
APD-Single Photon Counting Module (SPCM-AQRH-1X)
Software Packages:
- Imspector
- Lightbox (incl. Timebox - FLIM Phasor Plot Analysis)
- Picoquant SymPhoTime 64 (incl. analysis routines for FLIM, FCS, FLCS, FRET)
Objectives:
Olympus 40x, UPLFLN, 1.3NA Oil, 0.2mm WD
Olympus 60x, UPLSAPO 1.2NA water, 0.28mm WD
Olympus 100x, UPLSAPO 1.4NA Oil, 0.13mm WD
Book the system here