/people
/people
Dr. Vibor Laketa
Head of Infectious Diseases Imaging Platform
phone: +49 (0)6221 56-34410
e-mail: vibor.laketa@med.uni-heidelberg.de
Since 2018 Head of Infectious Diseases Imaging Platform (IDIP), Center for Integrative Infectious Disease Research (CIID), Heidelberg
Since 2013 Imaging platform coordinator in German Center for Infection Research (DZIF), Heidelberg, Germany
2008-2013 Staff Scientist, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
2006-2008 Postdoctoral fellow, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
2002-2006 PhD thesis, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
1997-2002 Diploma thesis, University of Zagreb, Croatia and Ludwig Institute for Cancer Research, Uppsala, Sweden.
Dr. Sylvia Olberg
phone: +49 (0)6221 56-4789
e-mail: sylvia.olberg@uni-heidelberg.de
Expertise:
point laser scanning confocal microscopy
TIRF microscopy
cell biology and parasitology
Dr. Severina Klaus
phone: +49 (0)6221 56-4789
e-mail: severina.klaus@med.uni-heidelberg.de
Expertise:
super resolution microscopy (STED, Airyscan)
live cell imaging
cell biology, virology, parasitology
Dr. Alessandro Ulivi
phone: +49 (0)6221 56-4789
e-mail: AlessandroFrancesco.Ulivi@med.uni-heidelberg.de
Expertise:
multiphoton microscopy, morphological profiling
live cell imaging
image anaylsis
Dr. Jessica Kehrer
phone: +49 (0)6221 56-32241
e-mail: jessica.kehrer@med.uni-heidelberg.de
Expertise:
spinning disc confocal microscopy
parasitology
Biosafety, lasersafety
IDIP associated experts
Dr. Bettina Stolp-Rastaetter
phone: +49 (0)6221 56-34631
e-mail: bettina.stolp@med.uni-heidelberg.de
Expertise:
multiphoton microscopy
sample preparation (optical clearing, cryostat sectioning)
virology
Dr. Giulia Mizzon
phone: +49 (0)6221 56-4834
e-mail: giulia.mizzon@med.uni-heidelberg.de
Expertise:
light microscopy
correlative light electron microscopy
electron microscopy sample preparation
virology
Jana Makroczyova
phone: +49 (0)6221 54-51230
e-mail: jana.makroczyova@bioquant.uni-heidelberg.de
Expertise:
correlative light electron microscopy
electron microscopy sample preparation
Dr. Annett Petrich
phone: +49 (0)6221 56-5008
e-mail: annett.petrich@med.uni-heidelberg.de
Expertise:
biophysical methods in live imaging
fluorescence lifetime microscopy
expansion microscopy
super resolution microscopy
cell biology, microbiology, virology
Steering Committee
Prof. Dr. Hans-Georg Kraeusslich
Director, Center for Integrative Infectious Disease Research (CIID), Heidelberg
Prof. Dr. Oliver T. Fackler
Head of Section Integrative Virology
Center for Integrative Infectious Disease Research (CIID), Heidelberg
Prof. Dr. Freddy Frischknecht
Head of Section Integrative Parasitology
Center for Integrative Infectious Disease Research (CIID), Heidelberg
Prof. Dr. Ralf Bartenschlager
Head of Molecular Virology Department
Center for Integrative Infectious Disease Research (CIID), Heidelberg
1. Spatial resolution of HIV-1 post-entry steps in resting CD4 T cells. Ananth S, Ambiel I, Schifferdecker S, Müller TG, Wratil PR, Mejias-Perez E, Kräusslich HG, Müller B, Keppler OT, Fackler OT.
Cell Rep. 2024 Mar 26;43(3):113941. doi: 10.1016/j.celrep.2024.113941. Epub 2024 Mar 12. PMID: 38478523
2. Probing Gag-Env dynamics at HIV-1 assembly sites using live-cell microscopy. Muecksch F, Klaus S, Laketa V, Müller B, Kräusslich H-G.
J Virol. 2024 Sep 17;98(9):e0064924. doi: 10.1128/jvi.00649-24. Epub 2024 Aug 13. PMID: 39136462
3. Expanding Insights: Harnessing Expansion Microscopy for Super-Resolution Analysis of HIV-1-Cell Interactions. Petrich A, Hwang GM, La Rocca L, Hassan M, Anders-Össwein M, Sonntag-Buck V, Heuser AM, Laketa V, Müller B, Kräusslich HG, Klaus S.
Viruses. 2024 Oct 15;16(10):1610. doi: 10.3390/v16101610. PMID: 39459943
4. Multiplex Microscopy Assay for Assessment of Therapeutic and Serum Antibodies against Emerging Pathogens. Sartingen N, Stürmer V, Kaltenböck M, Müller TG, Schnitzler P, Kreshuk A, Kräusslich HG, Merle U, Mücksch F, Müller B, Pape C, Laketa V.
Viruses. 2024 Sep 17;16(9):1473. doi: 10.3390/v16091473. PMID: 39339949
5.Phosphatidylinositol 4-Kinase III Alpha Governs Cytoskeletal Organization for Invasiveness of Liver Cancer Cells. Tran CS, Kersten J, Yan J, Breinig M, Huth T, Poth T, Colasanti O, Riedl T, Faure-Dupuy S, Diehl S, Verhoye L, Li TF, Lingemann M, Schult P, Ahlén G, Frelin L, Kühnel F, Vondran FWR, Breuhahn K, Meuleman P, Heikenwälder M, Schirmacher P, Bartenschlager R, Laketa V, Roessler S, Tschaharganeh DF, Sällberg M, Lohmann V.
Gastroenterology. 2024 Aug;167(3):522-537. doi: 10.1053/j.gastro.2024.04.009. Epub 2024 Apr 16.PMID: 38636680
6. Overcoming the egress block of Plasmodium sporozoites expressing fluorescently tagged circumsporozoite protein. Thieleke-Matos C, Walz K, Frischknecht F, Singer M.
Mol Microbiol. 2024 Mar;121(3):565-577. doi: 10.1111/mmi.15230. Epub 2024 Feb 23.PMID: 38396332
7. APEX-based proximity labeling in Plasmodium identifies a membrane protein with dual functions during mosquito infection. Kehrer J, Pietsch E, Ricken D, Strauss L, Heinze JM, Gilberger T, Frischknecht F.
PLoS Pathog. 2024 Dec 18;20(12):e1012788. doi: 10.1371/journal.ppat.1012788. eCollection 2024 Dec. PMID: 39693377
8. Nucleocapsid assembly drives Ebola viral factory maturation and dispersion. Vallbracht M, Bodmer BS, Fischer K, Makroczyova J, Winter SL, Wendt L, Wachsmuth-Melm M, Hoenen T, Chlanda P.
Cell. 2024 Dec 14:S0092-8674(24)01340-0. doi: 10.1016/j.cell.2024.11.024. PMID: 39742805
9. An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes. Chi H, Qu B, Prawira A, Richardt T, Maurer L, Hu J, Fu RM, Lempp FA, Zhang Z, Grimm D, Wu X, Urban S, Dao Thi VL.
EMBO Rep. 2024 Oct;25(10):4311-4336. doi: 10.1038/s44319-024-00236-0. Epub 2024 Sep 4. PMID: 39232200
10. Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells. Albanese M, Chen HR, Gapp M, Muenchhoff M, Yang HH, Peterhoff D, Hoffmann K, Xiao Q, Ruhle A, Ambiel I, Schneider S, Mejías-Pérez E, Stern M, Wratil PR, Hofmann K, Amann L, Jocham L, Fuchs T, Ulivi AF, Besson-Girard S, Weidlich S, Schneider J, Spinner CD, Sutter K, Dittmer U, Humpe A, Baumeister P, Wieser A, Rothenfusser S, Bogner J, Roider J, Knolle P, Hengel H, Wagner R, Laketa V, Fackler OT, Keppler OT.
Cell Rep Med. 2024 Apr 16;5(4):101483. doi: 10.1016/j.xcrm.2024.101483. Epub 2024 Apr 4. PMID: 38579727
11. Detection of Hepatitis C Virus Infection from Patient Sera in Cell Culture Using Semi-Automated Image Analysis. Schäfer N, Rothhaar P, Heuss C, Neumann-Haefelin C, Thimme R, Dietz J, Sarrazin C, Schnitzler P, Merle U, Pérez-Del-Pulgar S, Laketa V, Lohmann V.
Viruses. 2024 Nov 30;16(12):1871. doi: 10.3390/v16121871. PMID: 39772180
12. Turnover of PPP1R15A mRNA encoding GADD34 controls responsiveness and adaptation to cellular stress. Magg V, Manetto A, Kopp K, Wu CC, Naghizadeh M, Lindner D, Eke L, Welsch J, Kallenberger SM, Schott J, Haucke V, Locker N, Stoecklin G, Ruggieri A.
Cell Rep. 2024 Apr 23;43(4):114069. doi: 10.1016/j.celrep.2024.114069. Epub 2024 Apr 10. PMID: 38602876
13. Glucosylceramide in bunyavirus particles is essential for virus binding to host cells. Uckeley ZM, Duboeuf M, Gu Y, Erny A, Mazelier M, Lüchtenborg C, Winter SL, Schad P, Mathieu C, Koch J, Boulant S, Chlanda P, Maisse C, Brügger B, Lozach PY.
Cell Mol Life Sci. 2024 Feb 1;81(1):71. doi: 10.1007/s00018-023-05103-0.PMID: 38300320
1. Clearing of hemozoin crystals in malaria parasites enables whole-cell STED microscopy. Kehrer J, Pietsch E, Heinze J, Spielmann T, Frischknecht F.
J Cell Sci. 2023 Jan 1;136(1):jcs260399. doi: 10.1242/jcs.260399. Epub 2023 Jan 9.PMID: 36511329
2. Conformational change of Plasmodium TRAP is essential for sporozoite migration and transmission. Braumann F, Klug D, Kehrer J, Song G, Feng J, Springer TA, Frischknecht F.
EMBO Rep. 2023 Jul 5;24(7):e57064. doi: 10.15252/embr.202357064. Epub 2023 Jun 12.PMID: 37306042
3. Enhanced SARS-CoV-2 entry via UPR-dependent AMPK-related kinase NUAK2. Prasad V, Cerikan B, Stahl Y, Kopp K, Magg V, Acosta-Rivero N, Kim H, Klein K, Funaya C, Haselmann U, Cortese M, Heigwer F, Bageritz J, Bitto D, Jargalsaikhan S, Neufeldt C, Pahmeier F, Boutros M, Yamauchi Y, Ruggieri A, Bartenschlager R.
Mol Cell. 2023 Jul 20;83(14):2559-2577.e8. doi: 10.1016/j.molcel.2023.06.020. Epub 2023 Jul 7.PMID: 37421942
4. Comparison of HAV and HCV infections in vivo and in vitro reveals distinct patterns of innate immune evasion and activation. Colasanti O, Burm R, Huang HE, Riedl T, Traut J, Gillich N, Li TF, Corneillie L, Faure-Dupuy S, Grünvogel O, Heide D, Lee JY, Tran CS, Merle U, Chironna M, Vondran FFW, Esser-Nobis K, Binder M, Bartenschlager R, Heikenwälder M, Meuleman P, Lohmann V.
J Hepatol. 2023 Sep;79(3):645-656. doi: 10.1016/j.jhep.2023.04.023. Epub 2023 Apr 29.PMID: 37121436
5. The phenuivirus Toscana virus makes an atypical use of vacuolar acidity to enter host cells. Koch J, Xin Q, Obr M, Schäfer A, Rolfs N, Anagho HA, Kudulyte A, Woltereck L, Kummer S, Campos J, Uckeley ZM, Bell-Sakyi L, Kräusslich HG, Schur FK, Acuna C, Lozach PY.
PLoS Pathog. 2023 Aug 14;19(8):e1011562. doi: 10.1371/journal.ppat.1011562. eCollection 2023 Aug.PMID: 37578957
6. Tissue-like environments shape functional interactions of HIV-1 with immature dendritic cells. Gallucci L, Abele T, Fronza R, Stolp B, Laketa V, Sid Ahmed S, Flemming A, Müller B, Göpfrich K, Fackler OT.
EMBO Rep. 2023 Jun 5;24(6):e56818. doi: 10.15252/embr.202356818. Epub 2023 Apr 12.PMID: 37042686
7. Genomic profiling of HIV-1 integration in microglia cells links viral integration to the topologically associated domains. Rheinberger M, Costa AL, Kampmann M, Glavas D, Shytaj IL, Sreeram S, Penzo C, Tibroni N, Garcia-Mesa Y, Leskov K, Fackler OT, Vlahovicek K, Karn J, Lucic B, Herrmann C, Lusic M.
Cell Rep. 2023 Feb 28;42(2):112110. doi: 10.1016/j.celrep.2023.112110. Epub 2023 Feb 13.PMID: 36790927
8. ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells. Sadhu L, Tsopoulidis N, Hasanuzzaman M, Laketa V, Way M, Fackler OT.
Elife. 2023 May 10;12:e82450. doi: 10.7554/eLife.82450.PMID: 37162507
9. IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion. Klein S, Golani G, Lolicato F, Lahr C, Beyer D, Herrmann A, Wachsmuth-Melm M, Reddmann N, Brecht R, Hosseinzadeh M, Kolovou A, Makroczyova J, Peterl S, Schorb M, Schwab Y, Brügger B, Nickel W, Schwarz US, Chlanda P.
Cell Host Microbe. 2023 Apr 12;31(4):616-633.e20. doi: 10.1016/j.chom.2023.03.005. Epub 2023 Mar 31.PMID: 37003257
10. The Ebola virus VP40 matrix layer undergoes endosomal disassembly essential for membrane fusion. Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Lüchtenborg C, Fackler OT, Brügger B, Hoenen T, Nickel W, Schwarz US, Chlanda P.
EMBO J. 2023 Jun 1;42(11):e113578. doi: 10.15252/embj.2023113578. Epub 2023 Apr 21.PMID: 37082863
11. SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle. Zimmermann L, Zhao X, Makroczyova J, Wachsmuth-Melm M, Prasad V, Hensel Z, Bartenschlager R, Chlanda P.
Nat Commun. 2023 Nov 30;14(1):7894. doi: 10.1038/s41467-023-43666-5.PMID: 38036567
12. Progeny counter mechanism in malaria parasites is linked to extracellular resources. Stürmer VS, Stopper S, Binder P, Klemmer A, Lichti NP, Becker NB, Guizetti J.
PLoS Pathog. 2023 Dec 5;19(12):e1011807. doi: 10.1371/journal.ppat.1011807. eCollection 2023 Dec.PMID: 38051755
13. Plasmodium falciparum CRK4 links early mitotic events to the onset of S-phase during schizogony. Machado M, Klaus S, Klaschka D, Guizetti J, Ganter M.
mBio. 2023 Aug 31;14(4):e0077923. doi: 10.1128/mbio.00779-23. Epub 2023 Jun 22.PMID: 37345936
14. An Sfi1-like centrin-interacting centriolar plaque protein affects nuclear microtubule homeostasis. Wenz C, Simon CS, Romão TP, Stürmer VS, Machado M, Klages N, Klemmer A, Voß Y, Ganter M, Brochet M, Guizetti J.
PLoS Pathog. 2023 May 2;19(5):e1011325. doi: 10.1371/journal.ppat.1011325. eCollection 2023 May.PMID: 37130129
15. Malaria parasite centrins can assemble by Ca2+-inducible condensation. Voß Y, Klaus S, Lichti NP, Ganter M, Guizetti J.
PLoS Pathog. 2023 Dec 27;19(12):e1011899. doi: 10.1371/journal.ppat.1011899. eCollection 2023 Dec.PMID: 38150475
16. Endosomal egress and intercellular transmission of hepatic ApoE-containing lipoproteins and its exploitation by the hepatitis C virus. Pham MT, Lee JY, Ritter C, Thielemann R, Meyer J, Haselmann U, Funaya C, Laketa V, Rohr K, Bartenschlager R.
PLoS Pathog. 2023 Jul 28;19(7):e1011052. doi: 10.1371/journal.ppat.1011052. eCollection 2023 Jul.PMID: 37506130
17. Zika virus remodelled ER membranes contain proviral factors involved in redox and methylation pathways. Denolly S, Stukalov A, Barayeu U, Rosinski AN, Kritsiligkou P, Joecks S, Dick TP, Pichlmair A, Bartenschlager R.
Nat Commun. 2023 Dec 5;14(1):8045. doi: 10.1038/s41467-023-43665-6.PMID: 38052817
18. Zika virus prM protein contains cholesterol binding motifs required for virus entry and assembly. Goellner S, Enkavi G, Prasad V, Denolly S, Eu S, Mizzon G, Witte L, Kulig W, Uckeley ZM, Lavacca TM, Haselmann U, Lozach PY, Brügger B, Vattulainen I, Bartenschlager R.
Nat Commun. 2023 Nov 13;14(1):7344. doi: 10.1038/s41467-023-42985-x.PMID: 37957166
19. Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis. Pahmeier F, Lavacca T-M, Goellner S, Neufeldt CJ, Prasad V, Cerikan B, Rajasekharan S, Mizzon G, Haselmann U, Funaya C, Scaturro P, Cortese M, Bartenschlager R.
J Virol. 2023 Nov 30;97(11):e0087823. doi: 10.1128/jvi.00878-23. Epub 2023 Oct 31.PMID: 37905840
20. Dengue virus NS1 secretion is regulated via importin-subunit β1 controlling expression of the chaperone GRp78 and targeted by the clinical drug ivermectin. Denolly S, Guo H, Martens M, Płaszczyca A, Scaturro P, Prasad V, Kongmanas K, Punyadee N, Songjaeng A, Mairiang D, Pichlmair A, Avirutnan P, Bartenschlager R.
mBio. 2023 Oct 31;14(5):e0144123. doi: 10.1128/mbio.01441-23. Epub 2023 Sep 13.PMID: 37702492
1. SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB. Neufeldt CJ, Cerikan B, Cortese M, Frankish J, Lee JY, Plociennikowska A, Heigwer F, Prasad V, Joecks S, Burkart SS, Zander DY, Subramanian B, Gimi R, Padmanabhan S, Iyer R, Gendarme M, El Debs B, Halama N, Merle U, Boutros M, Binder M, Bartenschlager R.
Commun Biol. 2022 Jan 12;5(1):45. doi: 10.1038/s42003-021-02983-5.
2. The Orthobunyavirus Germiston Enters Host Cells from Late Endosomes. Windhaber S, Xin Q, Uckeley ZM, Koch J, Obr M, Garnier C, Luengo-Guyonnot C, Duboeuf M, Schur FKM, Lozach PY.
J Virol. 2022 Mar 9;96(5):e0214621. doi: 10.1128/jvi.02146-21. Epub 2022 Jan 12.
3. Temporal control of the integrated stress response by a stochastic molecular switch. Klein P, Kallenberger SM, Roth H, Roth K, Ly-Hartig TBN, Magg V, Aleš J, Talemi SR, Qiang Y, Wolf S, Oleksiuk O, Kurilov R, Di Ventura B, Bartenschlager R, Eils R, Rohr K, Hamprecht FA, Höfer T, Fackler OT, Stoecklin G, Ruggieri A.
Sci Adv. 2022 Mar 25;8(12):eabk2022. doi: 10.1126/sciadv.abk2022. Epub 2022 Mar 23.
4. Asynchronous nuclear cycles in multinucleated Plasmodium falciparum facilitate rapid proliferation. Klaus S, Binder P, Kim J, Machado M, Funaya C, Schaaf V, Klaschka D, Kudulyte A, Cyrklaff M, Laketa V, Höfer T, Guizetti J, Becker NB, Frischknecht F, Schwarz US, Ganter M.
Sci Adv. 2022 Apr;8(13):eabj5362. doi: 10.1126/sciadv.abj5362. Epub 2022 Mar 30.
5. Increased Sensitivity of SARS-CoV-2 to Type III Interferon in Human Intestinal Epithelial Cells. Metz-Zumaran C, Kee C, Doldan P, Guo C, Stanifer ML, Boulant S.
J Virol. 2022 Apr 13;96(7):e0170521. doi: 10.1128/jvi.01705-21. Epub 2022 Mar 9.
6. The FDA-Approved Drug Cobicistat Synergizes with Remdesivir To Inhibit SARS-CoV-2 Replication In Vitro and Decreases Viral Titers and Disease Progression in Syrian Hamsters. Shytaj IL, Fares M, Gallucci L, Lucic B, Tolba MM, Zimmermann L, Adler JM, Xing N, Bushe J, Gruber AD, Ambiel I, Taha Ayoub A, Cortese M, Neufeldt CJ, Stolp B, Sobhy MH, Fathy M, Zhao M, Laketa V, Diaz RS, Sutton RE, Chlanda P, Boulant S, Bartenschlager R, Stanifer ML, Fackler OT, Trimpert J, Savarino A, Lusic M.
mBio. 2022 Apr 26;13(2):e0370521. doi: 10.1128/mbio.03705-21. Epub 2022 Mar 1.
7. Phosphorylation of myosin A regulates gliding motility and is essential for Plasmodium transmission. Ripp J, Smyrnakou X, Neuhoff MT, Hentzschel F, Frischknecht F.
EMBO Rep. 2022 Jul 5;23(7):e54857. doi: 10.15252/embr.202254857. Epub 2022 May 4.
8. A Hepatitis C virus genotype 1b post-transplant isolate with high replication efficiency in cell culture and its adaptation to infectious virus production in vitro and in vivo. Heuss C, Rothhaar P, Burm R, Lee JY, Ralfs P, Haselmann U, Ströh LJ, Colasanti O, Tran CS, Schäfer N, Schnitzler P, Merle U, Bartenschlager R, Patel AH, Graw F, Krey T, Laketa V, Meuleman P, Lohmann V.
PLoS Pathog. 2022 Jun 28;18(6):e1010472. doi: 10.1371/journal.ppat.1010472. eCollection 2022 Jun.
9. Plasmodium sporozoite disintegration during skin passage limits malaria parasite transmission. Kehrer J, Formaglio P, Muthinja JM, Weber S, Baltissen D, Lance C, Ripp J, Grech J, Meissner M, Funaya C, Amino R, Frischknecht F.
EMBO Rep. 2022 Jul 5;23(7):e54719. doi: 10.15252/embr.202254719. Epub 2022 Apr 11.
10. Dual role of neddylation in transcription of hepatitis B virus RNAs from cccDNA and production of viral surface antigen. Qu B, Nebioglu F, Leuthold MM, Ni Y, Mutz P, Beneke J, Erfle H, Vondran FWR, Bartenschlager R, Urban S.
JHEP Rep. 2022 Aug 7;4(10):100551. doi: 10.1016/j.jhepr.2022.100551. eCollection 2022 Oct.
11. Direct Capsid Labeling of Infectious HIV-1 by Genetic Code Expansion Allows Detection of Largely Complete Nuclear Capsids and Suggests Nuclear Entry of HIV-1 Complexes via Common Routes. Schifferdecker S, Zila V, Müller TG, Sakin V, Anders-Össwein M, Laketa V, Kräusslich HG, Müller B.
mBio. 2022 Oct 26;13(5):e0195922. doi: 10.1128/mbio.01959-22. Epub 2022 Aug 16.
12. Monitoring Virus-Induced Stress Granule Dynamics Using Long-Term Live-Cell Imaging. Magg V, Klein P, Ruggieri A.
Methods Mol Biol. 2022;2428:325-348. doi: 10.1007/978-1-0716-1975-9_20.
1. Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies. Klein S, Wimmer BH, Winter SL, Kolovou A, Laketa V, Chlanda P.
Commun Biol. 2021 Jan 29;4(1):137.
2. Cone-shaped HIV-1 capsids are transported through intact nuclear pores. Zila V, Margiotta E, Turoňová B, Müller TG, Zimmerli CE, Mattei S, Allegretti M, Börner K, Rada J, Müller B, Lusic M, Kräusslich HG, Beck M.
Cell. 2021 Feb 18;184(4):1032-1046.e18.
3. A Versatile Reporter System To Monitor Virus-Infected Cells and Its Application to Dengue Virus and SARS-CoV-2. Felix Pahmeier, Christopher J. Neufeldt, Berati Cerikan, Vibhu Prasad, Costantin Pape, Vibor Laketa, Alessia Ruggieri, Ralf Bartenschlager, Mirko Cortese.
J Virol. 2021 Feb; 95(4): e01715-20.
4. Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells. Klein S, Wachsmuth-Melm M, Winter SL, Kolovou A, Chlanda P.
Methods Cell Biol. 2021;162:273-302.
5. Role of Clathrin Light Chains in Regulating Invadopodia Formation. Markus Mukenhirn, Francesco Muraca, Delia Bucher, Edgar Asberger, Elisa Cappio Barazzone, Elisabetta Ada Cavalcanti-Adam, Steeve Boulant.
Cells. 2021 Feb; 10(2): 451.
6. HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells. Müller TG, Zila V, Peters K, Schifferdecker S, Stanic M, Lucic B, Laketa V, Lusic M, Müller B, Kräusslich HG.
Elife. 2021 Apr 27;10:e64776.
7. From Multiplex Serology to Serolomics—A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome. Julia Butt, Rajagopal Murugan, Theresa Hippchen, Sylvia Olberg, Monique van Straaten, Hedda Wardemann, Erec Stebbins, Hans-Georg Kräusslich, Ralf Bartenschlager, Hermann Brenner, Vibor Laketa, Ben Schöttker, Barbara Müller, Uta Merle, Tim Waterboer.
Viruses. 2021 May; 13(5): 749.
8. Malaria parasites differentially sense environmental elasticity during transmission. Ripp J, Kehrer J, Smyrnakou X, Tisch N, Tavares J, Amino R, Ruiz de Almodovar C, Frischknecht F. EMBO Mol Med. 2021 Apr 9;13(4):e13933.
9. Fluorescent tagging of Plasmodium circumsporozoite protein allows imaging of sporozoite formation but blocks egress from oocysts. Singer M, Frischknecht F.
Cell Microbiol. 2021 May;23(5):e13321.
10. How Many Is Enough? - Challenges of Multinucleated Cell Division in Malaria Parasites. Simon CS, Stürmer VS, Guizetti J.
Front Cell Infect Microbiol. 2021 May 7;11:658616.
11. Prevalence of SARS-CoV-2 Infection in Children and Their Parents in Southwest Germany. Burkhard Tönshoff, Barbara Müller, Roland Elling, Hanna Renk, Peter Meissner, Hartmut Hengel, Sven F. Garbade, Meinhard Kieser, Kathrin Jeltsch, Jürgen Grulich-Henn, Julia Euler, Maximilian Stich, Kristine Chobanyan-Jürgens, Maria Zernickel, Aleš Janda, Lena Wölfle, Thomas Stamminger, Thomas Iftner, Tina Ganzenmueller, Christian Schmitt, Tessa Görne, Vibor Laketa, Sylvia Olberg, Anna Plaszczyca, Mirko Cortese, Ralf Bartenschlager, Constantin Pape, Roman Remme, Daniela Huzly, Marcus Panning, Sebastian Weigang, Sebastian Giese, Kevin Ciminski, Jakob Ankerhold, Georg Kochs, Martin Schwemmle, Rupert Handgretinger, Charlotte M. Niemeyer, Corinna Engel, Winfried V. Kern, Georg Friedrich Hoffmann, Axel R. Franz, Philipp Henneke, Klaus-Michael Debatin, Hans-Georg Kräusslich.
JAMA Pediatr. 2021 Jun; 175(6): 1–8.
12. Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils. Reif T, Dyckhoff G, Hohenberger R, Kolbe CC, Gruell H, Klein F, Latz E, Stolp B, Fackler OT.
Cell Rep Med. 2021 Jun 15;2(6):100317.
13. Advanced microscopy technologies enable rapid response to SARS-CoV-2 pandemic. Cortese M, Laketa V.
Cell Microbiol. 2021 Jul;23(7):e13319.
14. TMPRSS2 expression dictates the entry route used by SARS-CoV-2 to infect host cells. Koch J, Uckeley ZM, Doldan P, Stanifer M, Boulant S, Lozach PY.
EMBO J. 2021 Aug 16;40(16):e107821.
15. An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites. Simon CS, Funaya C, Bauer J, Voβ Y, Machado M, Penning A, Klaschka D, Cyrklaff M, Kim J, Ganter M, Guizetti J.
Life Sci Alliance. 2021 Sep 17;4(11):e202101199.
16. KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum-infected erythrocytes. Sanchez CP, Patra P, Chang SS, Karathanasis C, Hanebutte L, Kilian N, Cyrklaff M, Heilemann M, Schwarz US, Kudryashev M, Lanzer M.
Mol Microbiol. 2021 Sep 13.
17. Determinants in Nonstructural Protein 4A of Dengue Virus Required for RNA Replication and Replication Organelle Biogenesis. Cortese M, Mulder K, Chatel-Chaix L, Scaturro P, Cerikan B, Plaszczyca A, Haselmann U, Bartenschlager M, Neufeldt CJ, Bartenschlager R.
J Virol. 2021 Oct 13;95(21):e0131021.
18. Data fusion and smoothing for probabilistic tracking of viral structures in fluorescence microscopy images. Ritter C, Wollmann T, Lee JY, Imle A, Müller B, Fackler OT, Bartenschlager R, Rohr K.
Med Image Anal. 2021 Oct;73:102168.
19. Contribution of autophagy machinery factors to HCV and SARS-CoV-2 replication organelle formation. Twu WI, Lee JY, Kim H, Prasad V, Cerikan B, Haselmann U, Tabata K, Bartenschlager R.
Cell Rep. 2021 Nov 23;37(8):110049.
20. Convergent use of phosphatidic acid for hepatitis C virus and SARS-CoV-2 replication organelle formation. Tabata K, Prasad V, Paul D, Lee JY, Pham MT, Twu WI, Neufeldt CJ, Cortese M, Cerikan B, Stahl Y, Joecks S, Tran CS, Lüchtenborg C, V'kovski P, Hörmann K, Müller AC, Zitzmann C, Haselmann U, Beneke J, Kaderali L, Erfle H, Thiel V, Lohmann V, Superti-Furga G, Brügger B, Bartenschlager R.
Nat Commun. 2021 Dec 14;12(1):7276.
1. R-shaping Atlastin proteins act as central hubs to promote flavivirus replication and virion assembly. Christopher J. Neufeldt, Mirko Cortese, Pietro Scaturro, Berati Cerikan, Jeremy Wideman, Keisuke Tabata, Thaís Moraes, Olga Oleksiuk, Andreas Pichlmair, Ralf Bartenschlager.
Nat Microbiol. Nat Microbiol. 2019 Dec; 4(12): 2416–2429.
2. Real Time Analysis of Bovine Viral Diarrhea Virus (BVDV) Infection and Its Dependence on Bovine CD46. Riedel C, Chen HW, Reichart U, Lamp B, Laketa V, Rümenapf T.
Viruses. 2020 Jan 17;12(1):116.
3. Alterations of redox and iron metabolism accompany the development of HIV latency. Shytaj IL, Lucic B, Forcato M, Penzo C, Billingsley J, Laketa V, Bosinger S, Stanic M, Gregoretti F, Antonelli L, Oliva G, Frese CK, Trifunovic A, Galy B, Eibl C, Silvestri G, Bicciato S, Savarino A, Lusic M.
EMBO J. 2020 May 4;39(9):e102209.
4. Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses. Franziska Woelfl, Psylvia Léger, Nadia Oreshkova, Felix Pahmeier, Stefan Windhaber, Jana Koch, Megan Stanifer, Gleyder Roman Sosa, Zina M. Uckeley, Felix A. Rey, Steeve Boulant, Jeroen Kortekaas, Paul J. Wichgers Schreur, Pierre-Yves Lozach.
Viruses. 2020 Apr; 12(4): 400.
5. Regulatory myeloid cells paralyze T cells through cell-cell transfer of the metabolite methylglyoxal. Baumann T, Dunkel A, Schmid C, Schmitt S, Hiltensperger M, Lohr K, Laketa V, Donakonda S, Ahting U, Lorenz-Depiereux B, Heil JE, Schredelseker J, Simeoni L, Fecher C, Körber N, Bauer T, Hüser N, Hartmann D, Laschinger M, Eyerich K, Eyerich S, Anton M, Streeter M, Wang T, Schraven B, Spiegel D, Assaad F, Misgeld T, Zischka H, Murray PJ, Heine A, Heikenwälder M, Korn T, Dawid C, Hofmann T, Knolle PA, Höchst B.
Nat Immunol. 2020 May;21(5):555-566.
6. NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice. Psylvia Léger, Eliana Nachman, Karsten Richter, Carole Tamietti, Jana Koch, Robin Burk, Susann Kummer, Qilin Xin, Megan Stanifer, Michèle Bouloy, Steeve Boulant, Hans-Georg Kräusslich, Xavier Montagutelli, Marie Flamand, Carmen Nussbaum-Krammer, Pierre-Yves Lozach.
Nat Commun. 2020; 11: 3281.
7. Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells. Megan L. Stanifer, Carmon Kee, Mirko Cortese, Camila Metz Zumaran, Sergio Triana, Markus Mukenhirn, Hans-Georg Kraeusslich, Theodore Alexandrov, Ralf Bartenschlager, Steeve Boulant.
Cell Rep. 2020 Jul 7; 32(1): 107863.
8. A Non-Replicative Role of the 3′ Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation. Berati Cerikan, Sarah Goellner, Christopher John Neufeldt, Uta Haselmann, Klaas Mulder, Laurent Chatel-Chaix, Mirko Cortese, Ralf Bartenschlager.
Cell Rep. 2020 Jul 7; 32(1): 107859.
9. 3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway. Ilias Kounatidis, Megan L. Stanifer, Michael A. Phillips, Perrine Paul-Gilloteaux, Xavier Heiligenstein, Hongchang Wang, Chidinma A. Okolo, Thomas M. Fish, Matthew C. Spink, David I. Stuart, Ilan Davis, Steeve Boulant, Jonathan M. Grimes, Ian M. Dobbie, Maria Harkiolaki.
Cell. 2020 Jul 23; 182(2): 515–530.e17.
10. Evolutionarily distant I domains can functionally replace the essential ligand-binding domain of Plasmodium TRAP. Klug D, Goellner S, Kehrer J, Sattler J, Strauss L, Singer M, Lu C, Springer TA, Frischknecht F.
Elife. 2020 Jul 10;9:e57572.
11. SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography. Steffen Klein, Mirko Cortese, Sophie L. Winter, Moritz Wachsmuth-Melm, Christopher J. Neufeldt, Berati Cerikan, Megan L. Stanifer, Steeve Boulant, Ralf Bartenschlager, Petr Chlanda.
Nat Commun. 2020; 11: 5885.
12. Plasmodium falciparum parasites exit the infected erythrocyte after haemolysis with saponin and streptolysin O. Quadt KA, Smyrnakou X, Frischknecht F, Böse G, Ganter M.
Parasitol Res. 2020 Dec;119(12):4297-4302.
13. Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies. Mirko Cortese, Ji-Young Lee, Berati Cerikan, Christopher J. Neufeldt, Viola M.J. Oorschot, Sebastian Köhrer, Julian Hennies, Nicole L. Schieber, Paolo Ronchi, Giulia Mizzon, Inés Romero-Brey, Rachel Santarella-Mellwig, Martin Schorb, Mandy Boermel, Karel Mocaer, Marianne S. Beckwith, Rachel M. Templin, Viktoriia Gross, Constantin Pape, Christian Tischer, Jamie Frankish, Natalie K. Horvat, Vibor Laketa, Megan Stanifer, Steeve Boulant, Alessia Ruggieri, Laurent Chatel-Chaix, Yannick Schwab, Ralf Bartenschlager.
Cell Host Microbe. 2020 Dec 9; 28(6): 853–866.e5.
14. Microscopy‐based assay for semi‐quantitative detection of SARS‐CoV‐2 specific antibodies in human sera. Constantin Pape, Roman Remme, Adrian Wolny, Sylvia Olberg, Steffen Wolf, Lorenzo Cerrone, Mirko Cortese, Severina Klaus, Bojana Lucic, Stephanie Ullrich, Maria Anders‐Össwein, Stefanie Wolf, Berati Cerikan, Christopher J. Neufeldt, Markus Ganter, Paul Schnitzler, Uta Merle, Marina Lusic, Steeve Boulant, Megan Stanifer, Ralf Bartenschlager, Fred A. Hamprecht, Anna Kreshuk, Christian Tischer, Hans‐Georg Kräusslich, Barbara Müller, Vibor Laketa.
Bioessays. 2020 Dec 30 : 2000257.
15. HIV-1 infection of CD4 T cells impairs antigen-specific B cell function. Kaw S, Ananth S, Tsopoulidis N, Morath K, Coban BM, Hohenberger R, Bulut OC, Klein F, Stolp B, Fackler OT.
EMBO J. 2020 Dec 15;39(24):e105594.
16. Structures and distributions of SARS-CoV-2 spike proteins on intact virions. Ke Z, Oton J, Qu K, Cortese M, Zila V, McKeane L, Nakane T, Zivanov J, Neufeldt CJ, Cerikan B, Lu JM, Peukes J, Xiong X, Kräusslich HG, Scheres SHW, Bartenschlager R, Briggs JAG.
Nature. 2020 Dec;588(7838):498-502.
1. T cell receptor-triggered nuclear actin network formation drives CD4+ T cell effector functions. Tsopoulidis N, Kaw S, Laketa V, Kutscheidt S, Baarlink C, Stolp B, Grosse R, Fackler OT.
Sci Immunol. 2019 Jan 4;4(31):eaav1987.
2. HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex. Bejarano DA, Peng K, Laketa V, Börner K, Jost KL, Lucic B, Glass B, Lusic M, Müller B, Kräusslich HG.
Elife. 2019 Jan 23;8:e41800.
3. Immunofluorescence staining protocol for STED nanoscopy of Plasmodium-infected red blood cells. Mehnert AK, Simon CS, Guizetti. J. Mol Biochem Parasitol. 2019 Apr;229:47-52.
4. A novel interaction between dengue virus nonstructural protein 1 and the NS4A-2K-4B precursor is required for viral RNA replication but not for formation of the membranous replication organelle. Anna Płaszczyca, Pietro Scaturro, Christopher John Neufeldt, Mirko Cortese, Berati Cerikan, Salvatore Ferla, Andrea Brancale, Andreas Pichlmair, Ralf Bartenschlager.
PLoS Pathog. 2019 May; 15(5): e1007736.
5. Spatiotemporal Coupling of the Hepatitis C Virus Replication Cycle by Creating a Lipid Droplet- Proximal Membranous Replication Compartment. Lee JY, Cortese M, Haselmann U, Tabata K, Romero-Brey I, Funaya C, Schieber NL, Qiang Y, Bartenschlager M, Kallis S, Ritter C, Rohr K, Schwab Y, Ruggieri A, Bartenschlager R.
Cell Rep. 2019 Jun 18;27(12):3602-3617.e5.
6. Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production. Cortese M, Kumar A, Matula P, Kaderali L, Scaturro P, Erfle H, Acosta EG, Buehler S, Ruggieri A, Chatel-Chaix L, Rohr K, Bartenschlager R.
Cell Rep. 2019 May 28;27(9):2579-2592.e6.
7. Microtubule number and length determine cellular shape and function in Plasmodium Spreng B, Fleckenstein H, Kübler P, Di Biagio C, Benz M, Patra P, Schwarz US, Cyrklaff M, Frischknecht F.
EMBO J. 2019 Aug 1;38(15):e100984.
8. Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration Lucic B, Chen HC, Kuzman M, Zorita E, Wegner J, Minneker V, Wang W, Fronza R, Laufs S, Schmidt M, Stadhouders R, Roukos V, Vlahovicek K, Filion GJ, Lusic M.
Nat Commun. 2019 Sep 6;10(1):4059.
9. Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells. Zila V, Müller TG, Laketa V, Müller B, Kräusslich HG.
mBio. 2019 Nov 5;10(6):e02501-19.
10. Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures. Imle A, Kumberger P, Schnellbächer ND, Fehr J, Carrillo-Bustamante P, Ales J, Schmidt P, Ritter C, Godinez WJ, Müller B, Rohr K, Hamprecht FA, Schwarz US, Graw F, Fackler OT.
Nat Commun. 2019 May 13;10(1):2144.
1. Hepatitis C Virus Replication Depends on Endosomal Cholesterol Homeostasis. Ina Karen Stoeck, Ji-Young Lee, Keisuke Tabata, Inés Romero-Brey, David Paul, Philipp Schult, Volker Lohmann, Lars Kaderali, Ralf Bartenschlager.
J Virol. 2018 Jan 1; 92(1): e01196-17.
2. Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis. Delia Bucher, Felix Frey, Kem A. Sochacki, Susann Kummer, Jan-Philip Bergeest, William J. Godinez, Hans-Georg Kräusslich, Karl Rohr, Justin W. Taraska, Ulrich S. Schwarz, Steeve Boulant.
Nat Commun. 2018; 9: 1109.
3. Secretion of Hepatitis C Virus Replication Intermediates Reduces Activation of Toll-Like Receptor 3 in Hepatocytes. Grünvogel O, Colasanti O, Lee JY, Klöss V, Belouzard S, Reustle A, Esser-Nobis K, Hesebeck-Brinckmann J, Mutz P, Hoffmann K, Mehrabi A, Koschny R, Vondran FWR, Gotthardt D, Schnitzler P, Neumann-Haefelin C, Thimme R, Binder M, Bartenschlager R, Dubuisson J, Dalpke AH, Lohmann V.
Gastroenterology. 2018 Jun;154(8):2237-2251.e16.
4. Inter-subunit interactions drive divergent dynamics in mammalian and Plasmodium actin filaments. Douglas RG, Nandekar P, Aktories JE, Kumar H, Weber R, Sattler JM, Singer M, Lepper S, Sadiq SK, Wade RC, Frischknecht F.
PLoS Biol. 2018 Jul 16;16(7):e2005345.
5. Microscopy in Infectious Disease Research-Imaging Across Scales. Laketa V.
J Mol Biol. 2018 Aug 17;430(17):2612-2625.
6. Detailed Characterization of Early HIV-1 Replication Dynamics in Primary Human Macrophages. Bejarano DA, Puertas MC, Börner K, Martinez-Picado J, Müller B, Kräusslich HG.
Viruses. 2018 Nov 10;10(11):620.
/publications
/location
You can find as at the Heidelberg University campus area, within the Center for Integrative Infectious Disease Research (CIID), Building 344 (just across the botanical garden)
Im Neuenheimer Feld 344
69120 Heidelberg
Germany
IDIP is supported by following institutions and organisations
/contact
Vibor Laketa
Center for Integrative Infectious Diseases Research (CIID)
Infectious Diseases Imaging Platfrom (IDIP)
Im Neuenheimer Feld 344, 69120 Heidelberg, Germany
Tel: +49 6221 56 34410