Question 1

2021

Accepted Answer

1. Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies. Klein S, Wimmer BH, Winter SL, Kolovou A, Laketa V, Chlanda P. Commun Biol. 2021 Jan 29;4(1):137.  2. Cone-shaped HIV-1 capsids are transported through intact nuclear pores. Zila V, Margiotta E, Turoňová B, Müller TG, Zimmerli CE, Mattei S, Allegretti M, Börner K, Rada J, Müller B, Lusic M, Kräusslich HG, Beck M. Cell. 2021 Feb 18;184(4):1032-1046.e18.  3. A Versatile Reporter System To Monitor Virus-Infected Cells and Its Application to Dengue Virus and SARS-CoV-2. Felix Pahmeier, Christopher J. Neufeldt, Berati Cerikan, Vibhu Prasad, Costantin Pape, Vibor Laketa, Alessia Ruggieri, Ralf Bartenschlager, Mirko Cortese. J Virol. 2021 Feb; 95(4): e01715-20. 4. Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells. Klein S, Wachsmuth-Melm M, Winter SL, Kolovou A, Chlanda P. Methods Cell Biol. 2021;162:273-302. 5. Role of Clathrin Light Chains in Regulating Invadopodia Formation. Markus Mukenhirn, Francesco Muraca, Delia Bucher, Edgar Asberger, Elisa Cappio Barazzone, Elisabetta Ada Cavalcanti-Adam, Steeve Boulant. Cells. 2021 Feb; 10(2): 451. 
6. HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells. Müller TG, Zila V, Peters K, Schifferdecker S, Stanic M, Lucic B, Laketa V, Lusic M, Müller B, Kräusslich HG. Elife. 2021 Apr 27;10:e64776.  7. From Multiplex Serology to Serolomics—A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome. Julia Butt, Rajagopal Murugan, Theresa Hippchen, Sylvia Olberg, Monique van Straaten, Hedda Wardemann, Erec Stebbins, Hans-Georg Kräusslich, Ralf Bartenschlager, Hermann Brenner, Vibor Laketa, Ben Schöttker, Barbara Müller, Uta Merle, Tim Waterboer. Viruses. 2021 May; 13(5): 749.  8. Malaria parasites differentially sense environmental elasticity during transmission. Ripp J, Kehrer J, Smyrnakou X, Tisch N, Tavares J, Amino R, Ruiz de Almodovar C, Frischknecht F. EMBO Mol Med. 2021 Apr 9;13(4):e13933.   9. Fluorescent tagging of Plasmodium circumsporozoite protein allows imaging of sporozoite formation but blocks egress from oocysts. Singer M, Frischknecht F. Cell Microbiol. 2021 May;23(5):e13321. 10. How Many Is Enough? - Challenges of Multinucleated Cell Division in Malaria Parasites. Simon CS, Stürmer VS, Guizetti J. Front Cell Infect Microbiol. 2021 May 7;11:658616.  11. Prevalence of SARS-CoV-2 Infection in Children and Their Parents in Southwest Germany. Burkhard Tönshoff, Barbara Müller, Roland Elling, Hanna Renk, Peter Meissner, Hartmut Hengel, Sven F. Garbade, Meinhard Kieser, Kathrin Jeltsch, Jürgen Grulich-Henn, Julia Euler, Maximilian Stich, Kristine Chobanyan-Jürgens, Maria Zernickel, Aleš Janda, Lena Wölfle, Thomas Stamminger, Thomas Iftner, Tina Ganzenmueller, Christian Schmitt, Tessa Görne, Vibor Laketa, Sylvia Olberg, Anna Plaszczyca, Mirko Cortese, Ralf Bartenschlager, Constantin Pape, Roman Remme, Daniela Huzly, Marcus Panning, Sebastian Weigang, Sebastian Giese, Kevin Ciminski, Jakob Ankerhold, Georg Kochs, Martin Schwemmle, Rupert Handgretinger, Charlotte M. Niemeyer, Corinna Engel, Winfried V. Kern, Georg Friedrich Hoffmann, Axel R. Franz, Philipp Henneke, Klaus-Michael Debatin, Hans-Georg Kräusslich. JAMA Pediatr. 2021 Jun; 175(6): 1–8.  12. Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils. Reif T, Dyckhoff G, Hohenberger R, Kolbe CC, Gruell H, Klein F, Latz E, Stolp B, Fackler OT. Cell Rep Med. 2021 Jun 15;2(6):100317.  13. Advanced microscopy technologies enable rapid response to SARS-CoV-2 pandemic. Cortese M, Laketa V.Cell Microbiol. 2021 Jul;23(7):e13319. 14. TMPRSS2 expression dictates the entry route used by SARS-CoV-2 to infect host cells. Koch J, Uckeley ZM, Doldan P, Stanifer M, Boulant S, Lozach PY. EMBO J. 2021 Aug 16;40(16):e107821.  15. An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites. Simon CS, Funaya C, Bauer J, Voβ Y, Machado M, Penning A, Klaschka D, Cyrklaff M, Kim J, Ganter M, Guizetti J. Life Sci Alliance. 2021 Sep 17;4(11):e202101199.  16. KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum-infected erythrocytes. Sanchez CP, Patra P, Chang SS, Karathanasis C, Hanebutte L, Kilian N, Cyrklaff M, Heilemann M, Schwarz US, Kudryashev M, Lanzer M. Mol Microbiol. 2021 Sep 13.  17. Determinants in Nonstructural Protein 4A of Dengue Virus Required for RNA Replication and Replication Organelle Biogenesis. Cortese M, Mulder K, Chatel-Chaix L, Scaturro P, Cerikan B, Plaszczyca A, Haselmann U, Bartenschlager M, Neufeldt CJ, Bartenschlager R. J Virol. 2021 Oct 13;95(21):e0131021. 18. Data fusion and smoothing for probabilistic tracking of viral structures in fluorescence microscopy images. Ritter C, Wollmann T, Lee JY, Imle A, Müller B, Fackler OT, Bartenschlager R, Rohr K. Med Image Anal. 2021 Oct;73:102168.  19. Contribution of autophagy machinery factors to HCV and SARS-CoV-2 replication organelle formation. Twu WI, Lee JY, Kim H, Prasad V, Cerikan B, Haselmann U, Tabata K, Bartenschlager R. Cell Rep. 2021 Nov 23;37(8):110049. 20. Convergent use of phosphatidic acid for hepatitis C virus and SARS-CoV-2 replication organelle formation. Tabata K, Prasad V, Paul D, Lee JY, Pham MT, Twu WI, Neufeldt CJ, Cortese M, Cerikan B, Stahl Y, Joecks S, Tran CS, Lüchtenborg C, V'kovski P, Hörmann K, Müller AC, Zitzmann C, Haselmann U, Beneke J, Kaderali L, Erfle H, Thiel V, Lohmann V, Superti-Furga G, Brügger B, Bartenschlager R. Nat Commun. 2021 Dec 14;12(1):7276.

Question 2

2020

Accepted Answer

1. R-shaping Atlastin proteins act as central hubs to promote flavivirus replication and virion assembly. Christopher J. Neufeldt, Mirko Cortese, Pietro Scaturro, Berati Cerikan, Jeremy Wideman, Keisuke Tabata, Thaís Moraes, Olga Oleksiuk, Andreas Pichlmair, Ralf Bartenschlager. Nat Microbiol. Nat Microbiol. 2019 Dec; 4(12): 2416–2429.  2. Real Time Analysis of Bovine Viral Diarrhea Virus (BVDV) Infection and Its Dependence on Bovine CD46. Riedel C, Chen HW, Reichart U, Lamp B, Laketa V, Rümenapf T. Viruses. 2020 Jan 17;12(1):116.  3. Alterations of redox and iron metabolism accompany the development of HIV latency. Shytaj IL, Lucic B, Forcato M, Penzo C, Billingsley J, Laketa V, Bosinger S, Stanic M, Gregoretti F, Antonelli L, Oliva G, Frese CK, Trifunovic A, Galy B, Eibl C, Silvestri G, Bicciato S, Savarino A, Lusic M. EMBO J. 2020 May 4;39(9):e102209.  4. Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses. Franziska Woelfl, Psylvia Léger, Nadia Oreshkova, Felix Pahmeier, Stefan Windhaber, Jana Koch, Megan Stanifer, Gleyder Roman Sosa, Zina M. Uckeley, Felix A. Rey, Steeve Boulant, Jeroen Kortekaas, Paul J. Wichgers Schreur, Pierre-Yves Lozach. Viruses. 2020 Apr; 12(4): 400.  5. Regulatory myeloid cells paralyze T cells through cell-cell transfer of the metabolite methylglyoxal. Baumann T, Dunkel A, Schmid C, Schmitt S, Hiltensperger M, Lohr K, Laketa V, Donakonda S, Ahting U, Lorenz-Depiereux B, Heil JE, Schredelseker J, Simeoni L, Fecher C, Körber N, Bauer T, Hüser N, Hartmann D, Laschinger M, Eyerich K, Eyerich S, Anton M, Streeter M, Wang T, Schraven B, Spiegel D, Assaad F, Misgeld T, Zischka H, Murray PJ, Heine A, Heikenwälder M, Korn T, Dawid C, Hofmann T, Knolle PA, Höchst B. Nat Immunol. 2020 May;21(5):555-566.  6. NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice. Psylvia Léger, Eliana Nachman, Karsten Richter, Carole Tamietti, Jana Koch, Robin Burk, Susann Kummer, Qilin Xin, Megan Stanifer, Michèle Bouloy, Steeve Boulant, Hans-Georg Kräusslich, Xavier Montagutelli, Marie Flamand, Carmen Nussbaum-Krammer, Pierre-Yves Lozach. Nat Commun. 2020; 11: 3281.  7. Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells. Megan L. Stanifer, Carmon Kee, Mirko Cortese, Camila Metz Zumaran, Sergio Triana, Markus Mukenhirn, Hans-Georg Kraeusslich, Theodore Alexandrov, Ralf Bartenschlager, Steeve Boulant. Cell Rep. 2020 Jul 7; 32(1): 107863.  8. A Non-Replicative Role of the 3′ Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation. Berati Cerikan, Sarah Goellner, Christopher John Neufeldt, Uta Haselmann, Klaas Mulder, Laurent Chatel-Chaix, Mirko Cortese, Ralf Bartenschlager. Cell Rep. 2020 Jul 7; 32(1): 107859.  9. 3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway. Ilias Kounatidis, Megan L. Stanifer, Michael A. Phillips, Perrine Paul-Gilloteaux, Xavier Heiligenstein, Hongchang Wang, Chidinma A. Okolo, Thomas M. Fish, Matthew C. Spink, David I. Stuart, Ilan Davis, Steeve Boulant, Jonathan M. Grimes, Ian M. Dobbie, Maria Harkiolaki. Cell. 2020 Jul 23; 182(2): 515–530.e17.  10. Evolutionarily distant I domains can functionally replace the essential ligand-binding domain of Plasmodium TRAP. Klug D, Goellner S, Kehrer J, Sattler J, Strauss L, Singer M, Lu C, Springer TA, Frischknecht F. Elife. 2020 Jul 10;9:e57572.  11. SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography. Steffen Klein, Mirko Cortese, Sophie L. Winter, Moritz Wachsmuth-Melm, Christopher J. Neufeldt, Berati Cerikan, Megan L. Stanifer, Steeve Boulant, Ralf Bartenschlager, Petr Chlanda. Nat Commun. 2020; 11: 5885.  12. Plasmodium falciparum parasites exit the infected erythrocyte after haemolysis with saponin and streptolysin O. Quadt KA, Smyrnakou X, Frischknecht F, Böse G, Ganter M. Parasitol Res. 2020 Dec;119(12):4297-4302.  13. Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies. Mirko Cortese, Ji-Young Lee, Berati Cerikan, Christopher J. Neufeldt, Viola M.J. Oorschot, Sebastian Köhrer, Julian Hennies, Nicole L. Schieber, Paolo Ronchi, Giulia Mizzon, Inés Romero-Brey, Rachel Santarella-Mellwig, Martin Schorb, Mandy Boermel, Karel Mocaer, Marianne S. Beckwith, Rachel M. Templin, Viktoriia Gross, Constantin Pape, Christian Tischer, Jamie Frankish, Natalie K. Horvat, Vibor Laketa, Megan Stanifer, Steeve Boulant, Alessia Ruggieri, Laurent Chatel-Chaix, Yannick Schwab, Ralf Bartenschlager. Cell Host Microbe. 2020 Dec 9; 28(6): 853–866.e5.  14. Microscopy‐based assay for semi‐quantitative detection of SARS‐CoV‐2 specific antibodies in human sera. Constantin Pape, Roman Remme, Adrian Wolny, Sylvia Olberg, Steffen Wolf, Lorenzo Cerrone, Mirko Cortese, Severina Klaus, Bojana Lucic, Stephanie Ullrich, Maria Anders‐Össwein, Stefanie Wolf, Berati Cerikan, Christopher J. Neufeldt, Markus Ganter, Paul Schnitzler, Uta Merle, Marina Lusic, Steeve Boulant, Megan Stanifer, Ralf Bartenschlager, Fred A. Hamprecht, Anna Kreshuk, Christian Tischer, Hans‐Georg Kräusslich, Barbara Müller, Vibor Laketa. Bioessays. 2020 Dec 30 : 2000257. 15. HIV-1 infection of CD4 T cells impairs antigen-specific B cell function. Kaw S, Ananth S, Tsopoulidis N, Morath K, Coban BM, Hohenberger R, Bulut OC, Klein F, Stolp B, Fackler OT. EMBO J. 2020 Dec 15;39(24):e105594.  16. Structures and distributions of SARS-CoV-2 spike proteins on intact virions. Ke Z, Oton J, Qu K, Cortese M, Zila V, McKeane L, Nakane T, Zivanov J, Neufeldt CJ, Cerikan B, Lu JM, Peukes J, Xiong X, Kräusslich HG, Scheres SHW, Bartenschlager R, Briggs JAG. Nature. 2020 Dec;588(7838):498-502.

Question 3

2019

Accepted Answer

1. T cell receptor-triggered nuclear actin network formation drives CD4+ T cell effector functions. Tsopoulidis N, Kaw S, Laketa V, Kutscheidt S, Baarlink C, Stolp B, Grosse R, Fackler OT. Sci Immunol. 2019 Jan 4;4(31):eaav1987. 2. HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex. Bejarano DA, Peng K, Laketa V, Börner K, Jost KL, Lucic B, Glass B, Lusic M, Müller B, Kräusslich HG. Elife. 2019 Jan 23;8:e41800. 3. Immunofluorescence staining protocol for STED nanoscopy of Plasmodium-infected red blood cells. Mehnert AK, Simon CS, Guizetti. J. Mol Biochem Parasitol. 2019 Apr;229:47-52.  4. A novel interaction between dengue virus nonstructural protein 1 and the NS4A-2K-4B precursor is required for viral RNA replication but not for formation of the membranous replication organelle. Anna Płaszczyca, Pietro Scaturro, Christopher John Neufeldt, Mirko Cortese, Berati Cerikan, Salvatore Ferla, Andrea Brancale, Andreas Pichlmair, Ralf Bartenschlager. PLoS Pathog. 2019 May; 15(5): e1007736. 5. Spatiotemporal Coupling of the Hepatitis C Virus Replication Cycle by Creating a Lipid Droplet- Proximal Membranous Replication Compartment. Lee JY, Cortese M, Haselmann U, Tabata K, Romero-Brey I, Funaya C, Schieber NL, Qiang Y, Bartenschlager M, Kallis S, Ritter C, Rohr K, Schwab Y, Ruggieri A, Bartenschlager R. Cell Rep. 2019 Jun 18;27(12):3602-3617.e5.  6. Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production. Cortese M, Kumar A, Matula P, Kaderali L, Scaturro P, Erfle H, Acosta EG, Buehler S, Ruggieri A, Chatel-Chaix L, Rohr K, Bartenschlager R. Cell Rep. 2019 May 28;27(9):2579-2592.e6.  7. Microtubule number and length determine cellular shape and function in Plasmodium Spreng B, Fleckenstein H, Kübler P, Di Biagio C, Benz M, Patra P, Schwarz US, Cyrklaff M, Frischknecht F. EMBO J. 2019 Aug 1;38(15):e100984. 8. Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration Lucic B, Chen HC, Kuzman M, Zorita E, Wegner J, Minneker V, Wang W, Fronza R, Laufs S, Schmidt M, Stadhouders R, Roukos V, Vlahovicek K, Filion GJ, Lusic M. Nat Commun. 2019 Sep 6;10(1):4059.  9. Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells. Zila V, Müller TG, Laketa V, Müller B, Kräusslich HG. mBio. 2019 Nov 5;10(6):e02501-19.  
10. Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures. Imle A, Kumberger P, Schnellbächer ND, Fehr J, Carrillo-Bustamante P, Ales J, Schmidt P, Ritter C, Godinez WJ, Müller B, Rohr K, Hamprecht FA, Schwarz US, Graw F, Fackler OT. Nat Commun. 2019 May 13;10(1):2144.

Question 4

2018

Accepted Answer

1. Hepatitis C Virus Replication Depends on Endosomal Cholesterol Homeostasis. Ina Karen Stoeck, Ji-Young Lee, Keisuke Tabata, Inés Romero-Brey, David Paul, Philipp Schult, Volker Lohmann, Lars Kaderali, Ralf Bartenschlager. J Virol. 2018 Jan 1; 92(1): e01196-17.  2. Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis. Delia Bucher, Felix Frey, Kem A. Sochacki, Susann Kummer, Jan-Philip Bergeest, William J. Godinez, Hans-Georg Kräusslich, Karl Rohr, Justin W. Taraska, Ulrich S. Schwarz, Steeve Boulant. Nat Commun. 2018; 9: 1109.  3. Secretion of Hepatitis C Virus Replication Intermediates Reduces Activation of Toll-Like Receptor 3 in Hepatocytes. Grünvogel O, Colasanti O, Lee JY, Klöss V, Belouzard S, Reustle A, Esser-Nobis K, Hesebeck-Brinckmann J, Mutz P, Hoffmann K, Mehrabi A, Koschny R, Vondran FWR, Gotthardt D, Schnitzler P, Neumann-Haefelin C, Thimme R, Binder M, Bartenschlager R, Dubuisson J, Dalpke AH, Lohmann V. Gastroenterology. 2018 Jun;154(8):2237-2251.e16.  4. Inter-subunit interactions drive divergent dynamics in mammalian and Plasmodium actin filaments. Douglas RG, Nandekar P, Aktories JE, Kumar H, Weber R, Sattler JM, Singer M, Lepper S, Sadiq SK, Wade RC, Frischknecht F. PLoS Biol. 2018 Jul 16;16(7):e2005345. 5. Microscopy in Infectious Disease Research-Imaging Across Scales. Laketa V.J Mol Biol. 2018 Aug 17;430(17):2612-2625.  6. Detailed Characterization of Early HIV-1 Replication Dynamics in Primary Human Macrophages. Bejarano DA, Puertas MC, Börner K, Martinez-Picado J, Müller B, Kräusslich HG. Viruses. 2018 Nov 10;10(11):620.

/people

/people

Dr. Vibor Laketa

Head of Infectious Diseases Imaging Platform

Dr. Sylvia Olberg

​

phone: +49 (0)6221 56-4789

e-mail: sylvia.olberg@med.uni-heidelberg.de

Expertise:

point laser scanning confocal microscopy

TIRF microscopy

cell biology and parasitology

IDIP associated experts

Dr. Bettina Stolp-Rastaetter

phone: +49 (0)6221 56-34631

e-mail: bettina.stolp@med.uni-heidelberg.de

Expertise:

multiphoton microscopy

sample preparation (optical clearing, cryostat sectioning)

virology

Steffen Klein

​

phone: +49 (0)6221 54-51233

e-mail: steffen.klein@bioquant.uni-heidelberg.de

Expertise:

cryo light microscopy

correlative light electron microscopy

electron microscopy sample preparation

virology

Jessica Kehrer

​

phone: +49 (0)6221 56-6746

e-mail: jessica.kehrer@med.uni-heidelberg.de

Expertise:

spinning disc confocal microscopy

SPIM

3D model systems

parasitology

Steering Committee

Prof. Dr. Hans-Georg Kraeusslich

Director, Center for Integrative Infectious Disease Research (CIID), Heidelberg

Prof. Dr. Oliver T. Fackler

Head of Section Integrative Virology

Center for Integrative Infectious Disease Research (CIID), Heidelberg

Prof. Dr. Freddy Frischknecht

Head of Section Integrative Parasitology

Center for Integrative Infectious Disease Research (CIID), Heidelberg

Prof. Dr. Ralf Bartenschlager

Head of Molecular Virology Department

Center for Integrative Infectious Disease Research (CIID), Heidelberg

/publications

/location

/contact

Vibor Laketa