Nikon Ti2 with Crest spinning disc

Location:

CIID, BSL2, Room 024

Objectives:

 

1: Plan Apo Lambda D 10X/0,45/4,0

2: Plan Apo Lambda D 20X/0,8/0,8​

3: Plan Apo Lambda D 40X/0,95/0,21

4: Apo Lambda-S 40x WI/1.25/0,18

5: Plan Apo Lambda D 60X Oil/1,42/0,15

6: Plan Apo Lambda D 100x Oil/1,45/0,13

extra: Plan Apo Lambda S 25XC Sil 1.05/0,55

Lasers:

405 nm

446 nm

477 nm

520 nm

546 nm

638 nm

749 nm

LEDs:

385 nm

475 nm

550 nm

621 nm

Camera: 

Photometrics Kinetix 10.2 MP Mono

Back Illuminated sCMOS

3200 × 3200

6.5µm pixelsize

Filters (Emission):

1: pentaband 441/511/593/684/817

2: 438/24

3: 511/20

4: 595/31

5: 685/40

6: 819/44

7: 485/20

8: 560/25

Filters (dichroic):

1: pentaband DA/FI/TR/Cy5/Cy7 Celesta 391/477/549/639/741

2: CFP/YFP

Celesta 449/520

CrestOptics X-Light V3 spinning disc

Perfect Focus System (PFS)

NI-DAQ triggering

Environment control

Automated water dispenser

Flatfield illumination correction

AI-based denoising

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