Nikon TiE widefield microscope
LOCATION
CIID, BSL3 area, room U43
Objectives
10x NA=0.3, CFI Plan Fluor DL, Ph1, WD 15.2
20x NA=0.75, Plan Apo lambda, DIC N2, WD 1.0
40X NA=0.6, CFI S Plan Fluor ELWD ADM, Ph2, WD 3.6 – 2.8
40X NA=0.95, CFI Plan Apo Lambda, DIC N2, WD=0.25-0.16
60X Oil NA=1.4, CFI Plan Apo VC, DIC N2, WD 0.13
front-illuminated EM-CCD iXon 885 (1004 x 1002 pixels, 8um x 8um)
Environment control box (temperature, CO2)
fully motorized
Perfect Focus System (PFS)
external excitation filter wheel
feedback microscopy via Nikon JOBS
Filter settings
DAPI, ECFP, EGFP, EYFP, TRITC, Cy5
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