Nikon TiE widefield microscope

 

LOCATION

CIID, BSL3 area, room U43

Objectives

10x NA=0.3, CFI Plan Fluor DL, Ph1, WD 15.2

20x NA=0.75, Plan Apo lambda, DIC N2, WD 1.0

40X NA=0.6, CFI S Plan Fluor ELWD ADM, Ph2, WD 3.6 – 2.8

40X NA=0.95, CFI Plan Apo Lambda, DIC N2, WD=0.25-0.16

60X Oil NA=1.4, CFI Plan Apo VC, DIC N2, WD 0.13

front-illuminated EM-CCD iXon 885 (1004 x 1002 pixels, 8um x 8um)

Environment control box (temperature, CO2)

fully motorized

Perfect Focus System (PFS)

external excitation filter wheel

feedback microscopy via Nikon JOBS

Filter settings

DAPI, ECFP, EGFP, EYFP, TRITC, Cy5