VisitronSystems TIRF

Location

CIID, BSL2, EG, Room 023

Zeiss Axio Observer 7

PRIME-BSI-Express back-illuminated sCMOS (6.5um px size)

ASI MS-2000 xy stage, piezo z

Definite Focus 3

OKOLab laser safe environment control

Orbital-100 TIRF

Objectives:

A-Plan, 10x/0.25, Ph1

EC-Plan-NEOFLUAR, 20x/0.5

Plan-APOCHROMAT, 40x/1.3 Oil DIC (UV) 

alpha Plan-Apochromat 100x/1,46 Oil DIC

Widefield illumination:

CoolLED pE-4000

385nm, 490nm, 550nm, 660nm

Lasers:

405nm, 50mW

488nm, 100mW

561nm, 80mW

640nm, 150mW

Beamsplitters and emission filters:

1. Semrock Pentaband 408/504/581/667/762 for widefield

2. 405/488 TIRF illumination with 525/50 emission filter

3. GFP/RFP TIRF illumination with dual bandpass 515/30 - 650/80

4. CFP TIRF illumination(405LP) with 480/40 emission filter

5. Chroma Quadband 405/488/564/640 for TIRF