VisitronSystems TIRF

Location

CIID, BSL2, EG, Room 028

Zeiss Axiovert200M

Hamamatsu EMCCD 9100-50 (front illuminated, 8um px size)

ASI MS-2000 xy stage, piezo z

CRISP hardware-based autofocus

Environment control

Orbital-TIRF

Objectives:

A-Plan, 10x/0.25, Ph1

EC-Plan-NEOFLUAR, 20x/0.5

Plan-APOCHROMAT, 40x/1.3 Oil DIC (UV) 

alpha Plan-Apochromat 100x/1,46 Oil DIC

CoolLED pE-4000

widefield illumination

385nm, 490nm, 550nm, 660nm

Lasers:

405nm, 50mW

488nm, 100mW

561nm, 80mW

640nm, 40mW